CANdot Series GGold nanoparticles in life sciences

Plasmonic nanoparticles for optical read-out in applications in the visible wavelength range.

Gold nanoparticles exhibit an intensive red colour in aqueous dispersions caused by the so called localized surface plasmon resonance (LSPR). Light of a specific wavelength range enhances the characteristic frequency of the particles surface electrons, which undergo a collective oscillation (plasmon resonance). This phenomenon extremely amplifies the absorption of visible light of gold nanoparticles. A few femtomol of these particles on surfaces is sufficient for a visible detection. Furthermore, the strong light scattering of gold nanoparticles can be exploited e.g. in darkfield- or confocal microscopy techniques.

Citrate stabilized gold nanoparticles exhibit a strong affinity to molecules with positive net charge or positively charged functional groups, respectively. In particular, thiol groups bind as strong as quasi-covalent to gold nanoparticles.

The extremely high extinction coefficient in combination with the ease of biofunctionalization renders CANdot® Series G ideally for the generation of selective molecular markers (up to 5 orders of magnitude higher sensitivity than fluorescence-based detection techniques) e.g. for use in immunoassays. A quantitative assay read-out via absorption intensity is feasible, according to Lambert-Beer’s law. Alternatively, CANdot® Series G are offered pegylated, either neutral or with amine- or carboxyl groups for facile surface modifications. We also provide an optional customized functionalization service. Please visit CAN-Services

CANdot® Series G offer superior solutions for ultrasensitive optical read-outs and are characterized by the following features:

  • Standard particle diameters: 20 nm – 80 nm in 20 nm increments (additional sizes upon request)
  • Narrow size distribution (variation coefficient < 10%)
  • Shelf life > 12 month at 4°C
  • Ability to concentrate up to 0,2 µM
  • High stability even at high dilutions
  • Biofunctionalization possible
  • Quantitative absorption signal

Christian Supej

Christian Supej

distributor:

Order our CANdots Series G – Conjugation Kit direct and easy online.


Order our CANdots Series G – His Detect Kit direct and easy online.